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Coding

Part:BBa_K1045001

Designed by: iGEM Team Göttingen 2013   Group: iGEM13_Goettingen   (2013-06-24)

DarR ORF with inversed Pre- and Suffix

This is the ORF of DarR (Ms5346). DarR is a HTH-transcription factor from Mycobacterium smegmatis, which can bind its specific binding sequence (the DarR operator BBa_K1045000).

Zhang et al. (2013) showed that the binding efficiency of DarR to the operator is enhanced by cyclic di-AMP. Upon association with c-di-AMP, DarR binds strongly to the operator preventing transcription of the downstream gene. This way, transcription is controlled via c-di-AMP.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 581
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 594
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 31
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
n/aDarR ORF with inversed Pre- and Suffix